• Product NameTriton X-100
  • CasNo. 9002-93-1
  • MF(C2H4O)nC14H22O
  • MW250.37600
  • Purity
  • AppearanceClear oily liquid
  • Packing
  • Contact usInquiry

Product Details

CasNo: 9002-93-1

MF: (C2H4O)nC14H22O

Appearance: Clear oily liquid

Buy Quality Top Purity Triton X-100 9002-93-1 Competitive Price

  • Molecular Formula:(C2H4O)nC14H22O
  • Molecular Weight:250.37600
  • Appearance/Colour:Clear oily liquid 
  • Vapor Pressure:0mmHg at 25°C 
  • Melting Point:1oC 
  • Refractive Index:n20/D 1.491 
  • Boiling Point:6 °C 
  • Flash Point:250oC(lit.) 
  • PSA:29.46000 
  • Density:1.07 g/cm3 
  • LogP:3.77150 

Triton X-100(Cas 9002-93-1) Usage


Triton X-100, chemically known as polyethylene glycol p-(1,1,3,3-tetramethylbutyl)phenyl ether, is a nonionic surfactant. It consists of a hydrophilic polyethylene oxide chain and a lipophilic aromatic hydrocarbon group, specifically a 4-phenyl group. Its structure is a polyethylene glycol derivative with one of the terminal hydroxy groups converted into the corresponding p-(2,4,4-trimethylpentan-3-yl)phenyl ether.


Triton X-100 is widely utilized as a detergent in laboratories due to its ability to solubilize proteins, lyse cells for extraction of protein or organelles, and permeabilize cell membranes. Triton X-100 is commonly used in biochemical applications for its non-denaturing properties on proteins while denaturing cell membranes. However, its use as a surfactant raises environmental concerns because it is non-biodegradable and can inhibit certain microbial organisms. Biodegradation of Triton X-100 has been observed in aerobic and anaerobic municipal wastewater sludge and specific microbial organisms. Adsorption and biodegradation are the mechanisms responsible for Triton removal from the environment.

In addition, Triton X-100's insolubility in lipids is exploited for probing the structure of biological membranes. It is particularly useful for studying lipid bilayers with tight lipid packing. The Triton X-100-insoluble lipid fraction obtained from detergent extraction of eukaryotic cells consists of membranes rich in sphingolipids and cholesterol, which are often associated with lipid rafts. The degree of lipid insolubility depends on the stability of lipid-lipid interactions relative to lipid-detergent interactions and can vary based on factors such as lipid composition, detergent type, and temperature.


ChEBI: A poly(ethylene glycol) terminated with a 4-(2,4,4-trimethylpentan-3-yl) phenyl group at one end.


9002-93-1 Relevant articles

Temperature Dependence of Triton X-100 Micelle Size and Hydration

Kiril Streletzky and George D. J. Phillies

Langmuir 1995, 11, 1, 42–47

We studied the mutual diffusion coefficient of Triton X-100: water solutions for a series of Triton X-100 concentrations between 5 and 100 g/L and temperatures in the range 10-50 C.

A simple procedure for removal of Triton X-100 from protein samples

P.W. Holloway 2

, Analytical Biochemistry Volume 53, Issue 1, May 1973, Pages 304-308

A simple procedure is described for removal of Triton X-100 from protein samples by adsorption of the detergent on a commercial copolymer in bead form. The concentration of detergent can be lowered to approximately 0.01% with no loss or dilution of protein.

Permeabilization of microorganisms by Triton X-100

G.F. Miozzari, P. Niederberger, R. Hu¨tter

, Analytical Biochemistry Volume 90, Issue 1, 1 October 1978, Pages 220-233

Yeast cells are completely permeabilized by Triton X-100 concentrations of 0.05% (v/v), and permeabilization is independent of cell age and cell concentration. The treatment makes the cells freely diffusible for macromolecules up to molecular weights around 70,000.

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